|HPLC METHOD DEVELOPMENT|
|2-days Hands-on Short Course (Schedule)|
|Optimization of the HPLC Separation of Pharmaceutical Compounds|
Check your proficiency and decide whether you should attend this course or not.
|Below are four consecutive runs of an unknown mixture on a typical C18 column (150x4.6 mm) MeCN/Water 20/80 with different mobile phase pH (10 mM Phosphate buffer adjusted with HClO4).|
|Why does the retention of component A increase when the pH is lowered to 4 and then slightly decreases with further lowering the pH?|
|Why do basic components B and C switch their elution order between pH 2.5 and pH 2?|
|Why did the retention of component D decrease its retention when the pH was lowered from 6 to 2.5 ?|
|Why did the basic component E increase its retention when lowering the pH from
2.5 to 2?
|Why does the retention of peak F remain unchanged over the whole pH range 2 –6? (Hint: there are 3 explanations)|
|If you want an explanation for these effects, learn how to apply this knowledge for practical method development and learn to do it yourself|
|you should attend this course!|
|In two days participants will have 8 hours of lab work and 8 hours of lectures. You will be working on the development of a practical separation of an unknown mixture and learn how to select the proper starting conditions and how to modify them in the most optimal way to obtain a fast, reliable and rugged separation method.|
Email your name, company address and
billing details to Dr. Yuri Kazakevich
Somerset Marriott, Somerset NJ
|$1,250 per person: Please register early, maximum
participants per session
to allow hands-on experiments. Price includes: coffee breaks, lunch, books, and lab supplies.
20% DISCOUNT for GROUP of 3 or MORE
May 1-2, 2003