HPLC METHOD DEVELOPMENT
2-days Hands-on Short Course (Schedule)
Optimization of the HPLC Separation of Pharmaceutical Compounds

Check your proficiency and decide whether you should attend this course or not.

Below are four consecutive runs of an unknown mixture on a typical C18 column (150x4.6 mm) MeCN/Water 20/80 with different mobile phase pH (10 mM Phosphate buffer adjusted with HClO4).
Test.GIF (33915 bytes) Why does the retention of component A increase when the pH is lowered to 4 and then slightly decreases with further lowering the pH?
Why do basic components B and C switch their elution order between pH 2.5 and pH 2?
Why did the retention of component D decrease its retention when the pH was lowered from 6 to 2.5 ?
Why did the basic component E increase its retention when lowering the pH from
 2.5 to 2?
Why does the retention of peak F remain unchanged over the whole pH range 2 6? (Hint: there are 3 explanations)
If you want an explanation for these effects, learn how to apply this knowledge for practical method development and learn to do it yourself 
you should attend this course!
In two days participants will have 8 hours of lab work and 8 hours of lectures. You will be working on the development of a practical separation of an unknown mixture and learn how to select the proper starting conditions and how to modify them in the most optimal way to obtain a fast, reliable and rugged separation method.
TO REGISTER WHERE

Email  your name, company address and billing details to Dr. Yuri Kazakevich
kazakeyu@shu.edu

Somerset Marriott, Somerset NJ

PRICE WHEN
$1,250 per person: Please register early, maximum 12 participants per session
to allow hands-on experiments. Price includes: coffee breaks, lunch, books, and lab supplies.

20% DISCOUNT for GROUP of 3 or MORE

May 1-2, 2003

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